Mammals are composed of cells.Growing and harvesting mammalian cells outside of the human body and in the laboratory or factory to produce new drugs and medicines is one of the most exciting aspects of the modern life science industry.
These days, drug production based on cell culture is one of the fastest growing areas of biotechnology. Its rapid success owes much to the parallel development of technologies for the three key stages in cell culture growth: fermentation, harvesting and purification.
Harvesting is performed by separating the cell culture from the growing medium and several techniques are used to perform this delicate operation; centrifugation, microfiltration, depth filtration and filtration through absolute pore size membranes. Of the different techniques, centrifugation is the one most commonly used for scaling up from laboratory to factory production levels.
Most industrial applications use disc stack centrifuges to remove cells and cell debris from the nutrient broth. Disc stack centrifuges offer continuous operation, making their throughput consistent with the desire to limit the time for harvest operations.
Naturally, it is not quite as simple as it appears. Mammalian cells are very fragile organisms so. Although a disc stack centrifuge makes the job of separation relatively easy, the trick is to do so with minimal damage to the cells. Acceleration of the protein rich feed material takes a fraction of a second. But although speed is of the essence, it must not be at expense of destroying the highly shear-sensitive cell wall membrane which would release undesirable, intracellular proteins into the broth a process known as lysis.
By preventing additional lysis during acceleration, it is possible to increase the separator’s capacity while still achieving the required separation result.
Downstream purification of target proteins is also simplified and can be carried out using more compact equipment, thus generating significant savings in the process. The challenge, then, is to achieve maximum separation efficiency with minimal product disruption.
Just as cell culture has developed from relatively humble origins so has the equipment used to harvest it. In fact, from the earliest stages of cell culture science when Alfa Laval worked with industry leaders in the development of large scale cell culture fermentations, it soon became obvious that cell culture characteristics called for extremely gentle separator designs.
Several innovations have been made in the recent past.
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